Journal: Frontiers in Cell and Developmental Biology

Article Title: Down-Regulation of Lnc-CYP7A1-1 Rejuvenates Aged Human Mesenchymal Stem Cells to Improve Their Efficacy for Heart Repair Through SYNE1

doi: 10.3389/fcell.2020.600304

Figure Lengend Snippet: Expression profile of lncRNAs in young and old hBM-MSCs. (A) lncRNA expression in old (O) and young (Y) hBM-MSCs was determined by microarray analysis. (B) Differential lncRNA expression was validated by real-time qPCR. n = 5/group; ∗ P < 0.05 O vs. Y.

Article Snippet: Total RNA was isolated from hBM-MSCs after cultured in hypoxic conditions for 72 h. 12 × 135K lncRNA Expression Microarray (Arraystar, Rockville, MD, United States) was used to detected hBM-MSCs cDNA.

Techniques: Expressing, Microarray

Journal: Frontiers in Cell and Developmental Biology

Article Title: Down-Regulation of Lnc-CYP7A1-1 Rejuvenates Aged Human Mesenchymal Stem Cells to Improve Their Efficacy for Heart Repair Through SYNE1

doi: 10.3389/fcell.2020.600304

Figure Lengend Snippet: The expression of SYNE1 was inhibited by lnc-CYP7A1-1. (A) Gene co-expression networks were built to detect the interactions among lncRNAs and genes. Red color indicates expression up-regulated and green color indicates expression down-regulated. The dots represents genes and the diamond represents lncRNAs. (B) The expression of SYNE1 in old (O) and young (Y) hBM-MSCs was determined by microarray analysis. (C) The expression of SYNE1 in Old (O) and young (Y) hBM-MSCs was compared by real-time qPCR. (D) Old (O) hBM-MSCs were transfected by inhibition lentivirus (O-sh-CYP7A1) or control lentivirus (O-c), respectively. The expression of SYNE1 was compared by real-time qPCR. n = 5/group; * P < 0.05 O vs. Y, O-c vs. O-sh-CYP7A1.

Article Snippet: Total RNA was isolated from hBM-MSCs after cultured in hypoxic conditions for 72 h. 12 × 135K lncRNA Expression Microarray (Arraystar, Rockville, MD, United States) was used to detected hBM-MSCs cDNA.

Techniques: Expressing, Microarray, Transfection, Inhibition, Control

Journal: Cell Death & Disease

Article Title: PDPN + CAFs facilitate the motility of OSCC cells by inhibiting ferroptosis via transferring exosomal lncRNA FTX

doi: 10.1038/s41419-023-06280-3

Figure Lengend Snippet: Correlation between LncRNA FTX expression and clinicopathological parameters in oral squamous cell carcinomas.

Article Snippet: Exosomes derived from CAFs/Control and CAFs/sh-PDPN cells were subjected to a lncRNA microarray, and lncRNA sequencing was performed by RiboBio (China).

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: PDPN + CAFs facilitate the motility of OSCC cells by inhibiting ferroptosis via transferring exosomal lncRNA FTX

doi: 10.1038/s41419-023-06280-3

Figure Lengend Snippet: A , B The effect of lncRNA FTX transfection on FEN1 expression detected by qRT-PCR ( A ) and western blotting ( B ). Student’s t test for two-group comparison: ** P < 0.01. C Prediction of CpG island enrichment in FEN1 promoter region by the MethPrimer website. D , E The expression of FEN1 in WSU-HN6 and SCC15 cells treated with 5-Aza-dC detected by western blotting ( D ) and qRT-PCR ( E ). Student’s t test for two-group comparison: ** P < 0.01. F RPIseq databank predicts DNA demethylase TET2 are relevant to FTX using RF classifier and SVM classifier (RF > 0.5 & SVM > 0.5). G Potential binding sites for FTX within FEN1 promoter region verified using BLAST. H The enrichment of TET2 in FEN1 promoter region assessed by CHIP assay, and quantified by qRT-PCR. Student’s t test for two-group comparison: * P < 0.05; ** P < 0.01. I The binding relationship between FTX and TET2 examined by RNA pull-down. J The enrichment of FTX by TET2 assessed by RIP assay, and quantified by qRT-PCR. Student’s t test for two-group comparison: ** P < 0.01.

Article Snippet: Exosomes derived from CAFs/Control and CAFs/sh-PDPN cells were subjected to a lncRNA microarray, and lncRNA sequencing was performed by RiboBio (China).

Techniques: Transfection, Expressing, Quantitative RT-PCR, Western Blot, Comparison, Binding Assay

Journal: Cell Death & Disease

Article Title: PDPN + CAFs facilitate the motility of OSCC cells by inhibiting ferroptosis via transferring exosomal lncRNA FTX

doi: 10.1038/s41419-023-06280-3

Figure Lengend Snippet: A The expression of ferroptosis-related markers expression in FEN1-transfected OSCC cell lines detected by western blotting. B Representative immunohistochemical staining images of GPX4, SLC7A11, and ACSL4 in xenografts were shown. Scale bar, 100 μm. C The correlation between ACSL4 and FEN1 mRNA expression in fresh OSCC tissue samples ( n = 32). D The expression of FEN1 and ACSL4 in OSCC cells transfected with FEN1 and ACSL4 plasmid was measured by western blotting. E , F Schematic view of the reporter constructs containing different intervals of the ACSL4 regulatory region, generated for the mapping of the FEN1 responsive region ( E ). Luciferase activity was measured 48 h post-transfection ( F ). one-way ANOVA for multi-group comparisons: ** P < 0.01. G ChIP-qPCR assay of FEN1 and normal IgG in SCC15 and WSU-HN6 cells. Student’s t test for two-group comparison: ** P < 0.01. H Luciferase assay of increasing amount of FEN1 on the luciferase activity of pGL3-Basic and pGL3-ACSL4 plasmid in 293 T cells. Student’s t test for two-group comparison: * P < 0.05; ** P < 0.01. I Transwell assay was used to determine the effect of a FEN1 and ACSL4 plasmid transfection on migration and invasion of OSCC cells. Scale bar, 200 μm. one-way ANOVA for multi-group comparisons: * P < 0.05; ** P < 0.01. J A proposed model illustrating the role of PDPN + CAFs derived exosomal lncRNA FTX in regulating motility of OSCC cells by inhibiting ferroptosis through FTX/FEN1/ACSL4 signaling cascade.

Article Snippet: Exosomes derived from CAFs/Control and CAFs/sh-PDPN cells were subjected to a lncRNA microarray, and lncRNA sequencing was performed by RiboBio (China).

Techniques: Expressing, Transfection, Western Blot, Immunohistochemical staining, Staining, Plasmid Preparation, Construct, Generated, Luciferase, Activity Assay, ChIP-qPCR, Comparison, Transwell Assay, Migration, Derivative Assay